Founded in 1976 at the time the CIML was created, the Cytometry service functions with two engineers, Marc Barad (service manager), Sylvain Bigot and Laurence Borge. Cyrille Mionnet provides the scientific responsibility. The service is assisted by a steering committee made up of advanced users, which meets monthly. The service is reserved to CIML's users.
The missions of the service are:
- Cell sorting in biological conditions from any cell type in any kind of support (tube,1,5ml-15ml, 96 or 384 well plates). All sorting experiments are done in conditions insuring optimal cell viability.
- Quality control and maintenance of instruments
- Training of users. The training course consists of two modules: a theoretical module with basic notions of cytometry and a practical module on an analyser in small group. The novice user is then assisted at the beginning of his own experiment.
- Advice and assistance for flow cytometry experiments, data analysis and cell sorting.
- Evaluation of new technologies in term of analysis and cell sorting to maintain cytometry at his best level in the service.
- Development and optimization of the analyzers and sorters: Instruments modifications to reach optimum detection of new fluorochromes and fluorescent proteins.
The service has an online booking calendar and a network server for secure storage of data.
The instruments of the service are:
3 sorters
- Aria III upgrade modified with a yellow laser (4 lasers, 17 colors)
- Aria III SORP (5 lasers, 18 colors)
- Influx (4 lasers, 15 colors)
5 analyzers
- Canto II, 3 lasers, 8 colors
- Canto II, 3 lasers, 8 colors
- LSR II with a yellow laser, 4 lasers, 16 colors
- LSR II with a UV laser, 4 lasers, 18 colors
- LSR Fortessa X-20 5 lasers, 18 colors
8 stations for data analysis
- 5 Flowjo (MAC)
- 2 Diva (PC)
- 1 Infinicyt (PC)
Sorters
Sorters are available on working hours with a facility engineer.
Aria III upgrade
4 lasers, 17 colors
Aria III SORP
5 lasers, 18 colors
Influx
4 lasers, 15 colors
⇑ back
Analyzers
Analyzers are available 7/7 & 24/24 for those who have been trained by the service.
Canto II (Anakin)
3 lasers, 8 colors
Canto II (Yoda)
3 lasers, 8 colors
LSR II (561)
4 lasers, 16 colors
LSR II (UV)
4 lasers, 18 colors
5 lasers, 18 colors
