7F1

JEP131 521 bp


JEP132 532 bp
 


Composite sequence:

AGGGTTNNTG ATTCAAGACN TGNAGTAGCA AGGTGGTACC GNGATNCGTC
NGCACGGTGG NNACGCATTC CGNGCGAAGA NCAACGGGNG NCTCNTATGA
AGCGTTCANN GNNNANTTTC CCNCGGCCGG AAAGCGATCG CCAGGCGCTG
ATCGCGCTGC ATACGGGTGG AGAAAGATTA TCTNCCGGAG ATGACGCTGG
AGCAGAAGGT NGAGTGGCTC GATNCCCACA AGCTACACCC AGTTCNTGCG
TGAAAAAGTG GGCNTGAGCG AGCTGGCGAT CCGGTATTTC CAGCAGACCA
CCAACGACTT CCAGNCGGTG GGCATCGACG GCACCTCGTG CAGCGATGCG
CGCATCTGCG ATCTGCCGGG CCTCGGCGGC ATGAATCTGC CGCCGCTGGA
CGAAGAGTCG CAGGCGGATC TCGACGATCC TTACGTGTTC CACTTCCCGG
ACGGCAACGC CACGCTGGCG CGCCTGATGG TGCGCAAGCA TGATCCCGCG
GAGCGCCGGC CGGCAAAGAC A

GGCCAAATTC GACTACAGCC AGCTCGATCG GCCGGAGTCG CCGGTGAAGC
TGCGTTTAAA CAGCACCGGG CTGCACGCGG CCAACGTCGG CGACAAGGTG
GAAGTCACCT ATATGACCGG CGAGAAGATG ACCAAAGTGC GCGCCGGGCA
GGTGGTGATG GCCGGCTACA ACATGATGAT CCCGTACCTG GTGCCGGAGA
TGTCGCACGA GCAGCAGGAG GCGCTGAAGC AGAACGTCAA GGCGCCGCTG
GTGTACAGCA AGGTGGTGAT CCGCAACTGG CAGCCGTTCA TTAAGCTGGG
CGTGCACGAA ATTTATTCGC CGGCCGCGCC GTACAGCCGC GTGAAGCTGG
ACTACCCAGT GAGCATGGGC GGGTACCAGC ACCCGCGCGA TCCGGATCGG
CAATCGGCCT GCATATGGTG TCGTGCCGAC GCTGCGGCAG CGGCTCAGCC
CGCGCGAGCA GTCGCGCAAA GGGCCGCGCT GTTGTTGGCA CCCGTTCANG
TGACAACAGA TGTCCGGACA ATTGAGGCAT NT



BLASTX with this sequence gives only two hits (full results here):




>gi|11349969|pir||A83182   hypothetical protein PA3713 [imported] - Pseudomonas aeruginosa
           (strain PAO1)
          Length = 620

 Score =  157 bits (397), Expect(2) = 4e-57
 Identities = 73/131 (55%), Positives = 95/131 (72%)
 Frame = +1

Query: 523 AKFDYSQLDRPESPVKLRLNSTGLHAANVGDKVEVTYMTGEKMTKVRAGQVVMAGYNMMI 702
           A+FDYS+LD    PV+LRLNST +   N    V+V Y    ++ +VR    VMA YNMM+
Sbjct: 359 ARFDYSKLDLAGHPVRLRLNSTAVSVRNRAGGVDVGYSRAGRLHRVRGKHCVMACYNMMV 418

Query: 703 PYLVPEMSHEQQEALKQNVKAPLVYSKVVIRNWQPFIKLGVHEIYSPAAPYSRVKLDYPV 882
           PYL+ ++S EQ  AL QNVK PLVY+KV++RNWQ +  LG+HEIY+P  PYSR+KLD+PV
Sbjct: 419 PYLLRDLSEEQAHALSQNVKFPLVYTKVLLRNWQAWKTLGIHEIYAPTLPYSRIKLDFPV 478

Query: 883 SMGGYQHPRDP 915
            +G Y+HPRDP
Sbjct: 479 DLGSYRHPRDP 489


 Score = 85.9 bits (211), Expect(2) = 4e-57
 Identities = 49/141 (34%), Positives = 67/141 (47%)
 Frame = +3

Query: 78  RXTGXSYEAFXXXFPXAGKRSPGADRAAYGXXXXXXXXXXXXXXXXXXXPTSYTQFXREK 257
           R    S+ AF   FP + +    A  A Y                     TSY  +  + 
Sbjct: 207 RLNARSWRAFIGDFPLS-REDREALIALYESPRDYLAGKSVEEKETYLAKTSYRDYLLKN 265

Query: 258 VGXSELAIRYFQQTTNDFQXVGIDGTSCSDARICDXXXXXXXXXXXXDEESQADLDDPYV 437
           VG SE +++YFQ  +NDF  +G D    +DA                 EE+QA++D+PY+
Sbjct: 266 VGLSETSVKYFQGRSNDFSALGADALPAADAYAAGFPGFDALGLPQPSEEAQAEMDEPYI 325

Query: 438 FHFPDGNATLARLMVRKHDPA 500
           +HFPDGNA+LARLMVR   PA
Sbjct: 326 YHFPDGNASLARLMVRDLIPA 346




>gi|22977131|gb|ZP_00022954.1|   hypothetical protein [Ralstonia metallidurans]
          Length = 712

 Score = 97.4 bits (241), Expect(2) = 4e-24
 Identities = 53/134 (39%), Positives = 80/134 (59%), Gaps = 4/134 (2%)
 Frame = +1

Query: 484 ASMIPRSAGRQRQ----AKFDYSQLDRPESPVKLRLNSTGLHAANVGDKVEVTYMTGEKM 651
           + ++P  A  Q Q    A+FDYS+LDR  +PV++RLNST +          VTY     +
Sbjct: 439 SKLVPGVAAAQGQEIVGARFDYSKLDREGNPVRIRLNSTAIAIEPDVRITRVTYGWQGNL 498

Query: 652 TKVRAGQVVMAGYNMMIPYLVPEMSHEQQEALKQNVKAPLVYSKVVIRNWQPFIKLGVHE 831
            +V A  VV+AGY+MM+P+++P +    + AL+  VKAPLVY+KV + NW+ F  L    
Sbjct: 499 QRVEAAHVVVAGYDMMVPFILPSLPVPAKAALRACVKAPLVYTKVALDNWRAFDALKTWR 558

Query: 832 IYSPAAPYSRVKLD 873
           I++P   YS + L+
Sbjct: 559 IHAPTMAYSDLWLE 572


 Score = 35.4 bits (80), Expect(2) = 4e-24
 Identities = 25/90 (27%), Positives = 34/90 (37%)
 Frame = +3

Query: 228 TSYTQFXREKVGXSELAIRYFQQTTNDFQXVGIDGTSCSDARICDXXXXXXXXXXXXDEE 407
           T Y  F REK G     +R+ +  + D   +  DG + + A                D  
Sbjct: 356 TRYAVFLREKAGLGLPGLRFLRSRSQDAYALDADGITVAQAISIGLPAGAGMAAPAIDP- 414

Query: 408 SQADLDDPYVFHFPDGNATLARLMVRKHDP 497
            +          FPDGNATLAR +  K  P
Sbjct: 415 -RLGKSPASRLWFPDGNATLARALASKLVP 443

The transposon is thus inserted within a gene that encodes a homologue of hypothetical protein PA3713.

Direct homologues of this protein appear to be absent from sequenced bacteria (other than R. metallidurans; BLASTP results here). The most similar proteins are protoporphyrinogen oxidases, but the similarity is restricted almost entirely to an NAD binding site (residues 76-106; see the result from the ProfileScan Server), found also, for example in Thi4 domains (see here).

The protein in fact contains an amino oxidase domain (residues 83-620; see the entry for PA3713 at Pfam).

Protoporphyrinogen oxidases are protoheme biosynthetic enzyme. See for example Hansson & Hederstedt (1992).

Accepting the possibility that this protein is a protoporphyrinogen oxidase, STRING analysis suggests that it would be a (hemY) orthologue (see colour codes).

The current (09/02) Pseudomonas aeruginosa Community annotation for this gene is as a gene of unknown function, in a genomic context that is clearly distinct from that of hemY, with the downstream gene PA3714 (see also SWISSPROT/TrEMBL entry Q9HXS7) being a probable two-component response regulator.