7A8

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:


JEP131 530 bp


JEP132 99 bp before the next PstI site


Composite sequence:

NTGGGTNAGT ACTNGNANCN TNTGTGNGNN GNGCCNGGNT TNCGANCANT
GAGTGNCTGT ANNAGTTGCG NAGATGTACN NCGGGNCCAG TTNCGTGCGT
NCCNNCCTCG NTCCGTCGNN NGCNGGGNGT ATTTACAGAT AAATTTGGAT
CGTCTGTNCG GTGTCTTCAN NCGTGCAGTT CGGCGATCAG CGACAGCTGC
NANNTNGNTG CCGGCGAGTG ACGCCGCCAC CGCTGATTNC GTNNCCGTCG
CGTTCTACGC GAGCAGCGCT GGGTATGGCN NCGAAACAGC TTCAGGCTAT
CGCGCATAGA CCAGTGGCAG GCGGCGCGTT TACCCAGCAA TAGCCCGGCG
GCGGCTAGGA TCAGCGAACC GGTACACANC CGAGGTCAGG TAGCGAGCAT
CGGTGCCCAG CCGAACGGAT TTGCCGCATG AATGCTTCAT CCTGTAATGC
CTGGGTGCAG CCGCTGCCGC CCGGCACGCA CAGCACGTCA CAGCGCGCGN
GTCCGGCAGC NNGCGCAGCT GNTCTTAAAA 

TTTTGANAAC CATGAAGTGC AAGCCTTCCG ATTCGACATC CGCGCCGTCT
ACCGAAGCGA CGATGATTTC CGCGCCCGGC ATGCGGCGCA GAAACTGCA



BLASTX with this sequence gives a single significant hit (full results here):

>gi|16127189|ref|NP_421753.1| (NC_002696) ThiJ/PfpI family protein [Caulobacter crescentus]
          Length = 258

 Score = 68.6 bits (166), Expect = 4e-11
 Identities = 52/141 (36%), Positives = 68/141 (47%), Gaps = 4/141 (2%)
 Frame = -3

Query: 627 QFLRRMPGAEIIVASVDGADVESEGLHFMVXKILRXXXXXXXXARCDVLCVPGGSGCTQA 448
           Q L R+PGA++ VAS+ G ++E++GL F     L           CDVL VPGG G TQA
Sbjct: 57  QVLCRLPGAKVTVASLAGGEIEADGLVFARLPKLSDIET------CDVLIVPGGFGTTQA 110

Query: 447 LQDEAFMRQIR----SAGHRCSLPDLGCVPVR*S*PPPGYCWVNAPPATGLCAIA*SCFX 280
           + D  F+ +IR     A + CS+     V         G                 + F 
Sbjct: 111 MGDPDFIAEIRRLADGARYVCSVCTGSLVLA-----AAGLLKGKRAACHWAWRDQLALFG 165

Query: 279 AIPSAARVERDGXXISGGGVT 217
           A+P AARV RDG  I+GGGVT
Sbjct: 166 AVPDAARVARDGRYITGGGVT 186

The transposon is thus inserted within a gene that is homologous to the CC2959 gene, that encodes a DJ-1/PfpI family protein (see Pfam).

This family includes ThiJ a thiamine biosynthesis enzyme, the protease PfpI, as well as transcriptional regulators.

Thus, BLASTP with CC2959 (results here) identifies a number of functionally distinct proteins.

Interestingly, a human homologue (see BLAST2 results) DJ-1 (http://www.sciencemag.org/cgi/content/abstract/1077209)has very recently been shown to be involved in early onset parkinsonism and may play a rôle in the oxidative stress response .