7A8
/jep 132
TTTTGANAAC CATGAAGTGC AAGCCTTCCG ATTCGACATC CGCGCCGTCT
ACCGAAGCGA CGATGATTTC CGCGCCCGGC ATGCGGCGCA GAAACTGCAG
GCACGCGTCG GAAACTACGC CAACGCCGGC ACGCCCCTGG TGGCAGTTGG
TGGATGTGCA TTCGTTTTAT GTGCTCGGAT ATTTCGAAGA AACCAAGCTG
AAGCATATAA AAGAAGGCAA TAAAGCGGAC ATCGTTTTAT ATAACGGCAA
CACGCCGCTG CAGGCATGCA AGCTTCAGCT GCTCGAGTTC TATAGTGTCA
CCTAAATCGT ATGTGTATAG ATACATAAGG TTATGTATTA ATTGTANCCN
GTTTTAACGA CNNTATGTCC TATGGTGCAN TNTCANTACA ATTTGNTTTG
ATGCCTNCAN TTAATNCNNC NCGNCNCNTC ANACCNNTNA NNCNCNNTNA
NNNCTTTCTN TCNNNNCATN NNTNNNTANN NTTNANCTNT NNNNTNNTNN
TNNNAAT

TBLASTX shows that the sequence comes back into the vector

(results here) leaving:

TTTTGANAAC CATGAAGTGC AAGCCTTCCG ATTCGACATC CGCGCCGTCT
ACCGAAGCGA CGATGATTTC CGCGCCCGGC ATGCGGCGCA GAAACTGCAG
GCACGCGTCG GAAACTACGC CAACGCCGGC ACGCCCCTGG TGGCAGTTGG
TGGATGTGCA TTCGTTTTAT GTGCTCGGAT ATTTCGAAGA AACCAAGCTG
AAGCATATAA AAGAAGGCAA TAAAGCGGAC ATCGTTTTAT ATAACGGCAA
CACGCCGCTG CAGGCATGCA AGCTTCAGCT GCTCGAGTTC T

There are PstI sites at positions 99 and 262. That they
are real is confirmed by the TBLASTX results. Thus two
additional PstI fragments have been ligated together with
the Tn5-containing fragment.

This leaves:

TTTTGANAAC CATGAAGTGC AAGCCTTCCG ATTCGACATC CGCGCCGTCT
ACCGAAGCGA CGATGATTTC CGCGCCCGGC ATGCGGCGCA GAAACTGCA