7E7

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:


JEP131 500 bp


JEP132 24 bp then a Pst I site


Composite sequence:

CANCTGTGTA CACCTCCGAG GTGTCAACAG CGCGCTGCGA CCCTGCAGCC
GGTCGNTAGC GTCGGAATNT GNTGAGTGGA GCAGATGATG CAATGCGCTG
ATGCCCTTGC TGGATCAGAT GACGGTCGCC AGCCAGGCGC CGTAGCGATC
GTCGAGCGCC ACGCAGCGTG TTTCGAAGCC CGGCAGCGTG CGGTTGATCA
GCACCATGCC CGGGATCTGT TGCATCCAGC CGCAAAGTTC CTGATCGCTC
AGCCTTTTGG CGTGCACCAC CAGCGCTTCG CAGCGGTGCC GCACCAGCTG
TTCAATCGCC TGGCGCTCTT TTTCGGCGTC GTGGTAACCG TTGCCAATCA
GCAGAAAATT ATGGGTGGCG TAGGCCACCT GCTCGACCGC TTTGACCATT
GCGCCGAAGA AGGGATCGGA AACGTCGGAG ACGATCAGGC CCAGCGTCTC
GGTGGATTGC NNCGCCATCG CCCGTGCGTG GGGAANGNGA NNNNNANNGG

AGAAGGCGTT AGGGTGATAC TGCA


The top match for BLASTX (full results here) is:



>gi|22127059|ref|NP_670482.1|   (NC_004088) repressor of galETK operon [Yersinia pestis KIM]
          Length = 363

 Score =  218 bits (554), Expect = 1e-56
 Identities = 116/162 (71%), Positives = 126/162 (77%), Gaps = 1/162 (0%)
 Frame = -3

Query: 522 QYHPNAFSXXXXXPHARAMAXQSTETLGLIVSDVSDPFFGAMVKAVEQVAYATHNFLLIG 343
           QYHPNA        +ARA+A QSTET+G+IVSDVSDPFFGAMVKAVEQVAYAT NFLLIG
Sbjct: 67  QYHPNA--------NARALAQQSTETVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIG 118

Query: 342 NGYHDAEKERQAIEQLVRHRCEALVVHAKRLSDQELCGWMQQIPGMVLINRTLPGFETRC 163
           NGYHDAEKERQAIEQL+RHRC ALVVHAK+L D EL   M+QIPGMVLINRTLPGFE RC
Sbjct: 119 NGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRC 178

Query: 162 VALDDRYGAWLATVI*SSKGIXXXXXX-XXXXXSDAXDRLQG 40
           +ALDDRYGAWLAT     +G             SDA DR+QG
Sbjct: 179 IALDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQG 220

The transposon is thus inserted within a gene encoding a homologue of a Galactose operon repressor (see for example the E. coli protein and the entry for galR at EcoGene).