7E7
/jep 132
AGAAGGCGTT AGGGTGATAC TGCAGCTGCG GCAGGGGCAT CCTATGCCAT
CACCACCGTG CTCACTTACC TGATTACCGC CGTGGGCGCC GTGGTTGCGC
TGGGATCGCT CGGCGTCTCC TGGGACAAAC TGCAATGGCT GGCCGCCGGC
CTGACGGTCG GGCTGGGCTT CGGCCTGCAG GCCTCTGCCG AAGCATTGGC
TGAAGAGGTG AACGGCGAAG TCGTGCTGCA GCACCCGGAT AGTGTGCTCG
TCGACGGTAT AGGCGTGGAA CAGATCGAAC TGCATCTGCC CGACGATCTT
GCCCCACTGC GGCATATAAG CCCACAGCAC NCTGTGACGG TGCATCGGCA
CCAGCGCGCG CGACACCGCG CCGGGGTGGC GCAGGATGGC ATAAACAGAT
CGCGCGCTTC CGGGATGGTG ACAGCGGCTG TTTCANGTGA CGCGCGCGTG
CCACTGCCAC GTGGTGAATA AATGCTTTGA TC

The sequence contains several Pst I sites, giving just

AGAAGGCGTT AGGGTGATAC TGCA

That this is the case is confirmed by BLASTX using the composite sequence:

CANCTGTGTA CACCTCCGAG GTGTCAACAG CGCGCTGCGA CCCTGCAGCC
GGTCGNTAGC GTCGGAATNT GNTGAGTGGA GCAGATGATG CAATGCGCTG
ATGCCCTTGC TGGATCAGAT GACGGTCGCC AGCCAGGCGC CGTAGCGATC
GTCGAGCGCC ACGCAGCGTG TTTCGAAGCC CGGCAGCGTG CGGTTGATCA
GCACCATGCC CGGGATCTGT TGCATCCAGC CGCAAAGTTC CTGATCGCTC
AGCCTTTTGG CGTGCACCAC CAGCGCTTCG CAGCGGTGCC GCACCAGCTG
TTCAATCGCC TGGCGCTCTT TTTCGGCGTC GTGGTAACCG TTGCCAATCA
GCAGAAAATT ATGGGTGGCG TAGGCCACCT GCTCGACCGC TTTGACCATT
GCGCCGAAGA AGGGATCGGA AACGTCGGAG ACGATCAGGC CCAGCGTCTC
GGTGGATTGC NNCGCCATCG CCCGTGCGTG GGGAANGNGA NNNNNANNGG

AGAAGGCGTT AGGGTGATAC TGCAGCTGCG GCAGGGGCAT CCTATGCCAT
CACCACCGTG CTCACTTACC TGATTACCGC CGTGGGCGCC GTGGTTGCGC
TGGGATCGCT CGGCGTCTCC TGGGACAAAC TGCAATGGCT GGCCGCCGGC
CTGACGGTCG GGCTGGGCTT CGGCCTGCAG GCCTCTGCCG AAGCATTGGC
TGAAGAGGTG AACGGCGAAG TCGTGCTGCA GCACCCGGAT AGTGTGCTCG
TCGACGGTAT AGGCGTGGAA CAGATCGAAC TGCATCTGCC CGACGATCTT
GCCCCACTGC GGCATATAAG CCCACAGCAC NCTGTGACGG TGCATCGGCA
CCAGCGCGCG CGACACCGCG CCGGGGTGGC GCAGGATGGC ATAAACAGAT
CGCGCGCTTC CGGGATGGTG ACAGCGGCTG TTTCANGTGA CGCGCGCGTG
CCACTGCCAC GTGGTGAATA AATGCTTTGA TC


that gives (full results here):



>gi|22127059|ref|NP_670482.1|   (NC_004088) repressor of galETK operon [Yersinia pestis KIM]
 
          Length = 363

 Score =  221 bits (564), Expect = 2e-57
 Identities = 118/166 (71%), Positives = 129/166 (77%), Gaps = 1/166 (0%)
 Frame = -2

Query: 534 LPQLQYHPNAFSXXXXXPHARAMAXQSTETLGLIVSDVSDPFFGAMVKAVEQVAYATHNF 355
           + QLQYHPNA        +ARA+A QSTET+G+IVSDVSDPFFGAMVKAVEQVAYAT NF
Sbjct: 63  MEQLQYHPNA--------NARALAQQSTETVGMIVSDVSDPFFGAMVKAVEQVAYATGNF 114

Query: 354 LLIGNGYHDAEKERQAIEQLVRHRCEALVVHAKRLSDQELCGWMQQIPGMVLINRTLPGF 175
           LLIGNGYHDAEKERQAIEQL+RHRC ALVVHAK+L D EL   M+QIPGMVLINRTLPGF
Sbjct: 115 LLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGF 174

Query: 174 ETRCVALDDRYGAWLATVI*SSKGIXXXXXX-XXXXXSDAXDRLQG 40
           E RC+ALDDRYGAWLAT     +G             SDA DR+QG
Sbjct: 175 EPRCIALDDRYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQG 220


i.e. the similarity stops abruptly at a position (528) almost
exactly corresponding to the predicted Pst I site (524).