10H4

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:

JEP131 572 bp

JEP132 562 bp
 
Composite sequence:


NGNCGGNTNA TNCNNNCCTG CAACNNTCGN ATACNANGNA NTNNNNGNNA
NACNGCNGNA GCNNCTATGT CGGGATAACC AGCCTTCNGN ACNAACTNCA
GGNNATGGTG TAGCANATAG CAGAACAGTC CGANCATNNC CAGCCCTNNN
GCNGGCAAGN TGGTAAANCG NCGCATGAAC ATCGCCNTGG CCNGNNCTGG
ANTGACTGGT GAAACGAAAA TGTCTTCCGA AGGGTGGATC CCGTTTAGCA
TTGGCCAAGG TGTCCCATCG TCAATGTNGA GATCGCGGCG TTCGGTGNCC
AGCATCACCA GATCGGCATG CTTGACGATC GGGCTGGTGG TGGCTGGAAG
GCCGAATTTA NAACGAATAA CAAAATCAAT ATTATTTTCA GATGCCGCGA
TAGTCGGGAA TTAACTTTTT AAGCGGTGAT GGAATATCTT TGCAATATGC
TTCGTGCGCG TCATGCTAGT AATGCTTCAA AAGCAAAACT AGGCGGGACG
ATTTTACTGG CAATGACACA ATGCTGCGCC ACGCTATAGA ATTCAGGAAT
GTGACCATGA AGCGGGNTCA NA

GCGNGCNTTC ATGAGATAAG GCTTGCGCTA TATCTTCAAT GCAAATAGCG
TCAACGGTTG GCGCTTCGTA ATCAAAGCGT TTACCCGTAA AGGTTAAAAT
CCAAGACATA ATTATTCTCC ACACAGTTTT TGGCAACACT TCACCAAATA
CCCCATCGCT GGGGTATTTG AGGCTGTATT ACTTATTTAA AAAATCAGGC
TTTAAAATTA CCGATAAAGG TTTCTACTGA GCCGCCATCG AATTTATCGA
TCAGTAGATC GCGAAACTCG TTGGCAATTG CCTCTTCCTG CGCTTCAAGT
TGAACAATGC NCAAGACGAA AAGCGGCTCG TCACTTTTCA GCAGGCTATT
GCGAAGGCTG AACTTNGCGT TCCCCGAGGC CTTATACGGG GTGCAGGTGA
ATTCGAATGC AACCGGCATC ACGTCTTTGC TTGTGGCTTC ATGCTTTGCA
TGAGNGATTT TTACCGCTGA NTACCGCTTC ATGATCTGTT CTGACNTGCT
AATGCTNCGC GNAACGACCG GAGCCNCAGA TGCATGTTAC CNNGGATNAA
NGTTCAGATA CN



BLASTX with this sequence (full results here) gives as most significant hit :


>gi|16130293|ref|NP_416862.1| (NC_000913) orf, hypothetical protein [Escherichia coli K12]
           Length = 187


 Score = 62.4 bits (150), Expect(2) = 2e-29
 Identities = 40/99 (40%), Positives = 57/99 (57%), Gaps = 4/99 (4%)
 Frame = -1

Query: 681 MSWILTFTGKRFDYEAPTVDAICIEDIAQALSHEXXL*XRFMVTFLNSIA-WRSIVSLPV 505
           MS+I TF+GK F Y+    D I I DIA +LS+        +  F   ++ + S+    V
Sbjct: 10  MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSN--------ICRFAGHLSHFYSVAQHAV 61

Query: 504 KSSRLV---LLLKHY*HDAHEAYCKDIPSPLKKLIPDYR 397
             S+LV      +   HDA EAYC+DIP+PLK+L+PDY+
Sbjct: 62  LCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYK 100

The transposon is thus inserted within with a gene that encodes a homologue of an E. coli hypothetical protein that, depending on the prediction, is either 178 (YFDR) or 187 (b2361) amino acids long).

There is no functional information available for this protein or its homologues (see BLASTP result).

b2361 does not contain any identifiable Pfam domains.

STRING analysis suggests that neither b2361/yfdR nor its orthologs occur repeatedly with certain genes in one gene cluster.

Colibri shows that b2361/yfdR is surrounded by genes of unknown function, with the upstream gene being yfdQ. See also the entry for yfdR at EcoGene.