10H1

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:


JEP131 504 bp


JEP132 405 bp


 
Composite sequence:


AGAGCTNTGG GGCGATCGTT TCGGCGGGGG GCGCGAGGCG TTATTGCGCG
AGGGCGCTGC CGTTCAACGA CTGCTGACGC TAACGTTTTC CGCCAAAGAG
AGCCTGTTCA AGGCGCTTTA TCCGCAGGTG CGCTGTTACT TCGATTTTCT
CGATGCGCGC ATGGTCGCCG TGGATACGCA GCGGCAGACG TTTGTATTAG
CGTTGCTTAA GACCTTGACG CCAAATTGCC CCGCCGGCCG CCGGTTTAAC
GGGCGTTTTT GGCGTGAAGG TGACGATGTC ACGACTTTTA TTTTTTGCTA
AAACATAATA ATAACAGTGT GTTATTTCTT GCGGCGCCGT AACGCGGATG
TGCCGCGGCG GGCGGAAAGA TTCATTCATC GGAGATTGAC CCACGGTGGG
GTTCCGGTGT AGTTTATTCT CCTTTTATAG CTAATGATAA TTATTCGCAT
AAACATTATC ATTGTTAGGC GCAACAAAGG AGTGTANNNG GGGANNAAAA
GGGG

CCNANTNGGT TGNTGGCACA CTGACGACAG AAAACCAAAC CTTCCCCGGG
TTTGTGTACG CCGAACAATC GACCTTTTTG TACCGGTCCG AATTCCGGTC
CCTCTCCGCC GACGGCGTGT TTGAGCGTAT CGAAACGCCG GCGTTCGGCG
GCGAGCAGGA AGGCAGCGCG CTGGCGCAGC ATATTCGCCA GGCGCTGGCG
CGCGCCAAAG CCGCCGGCCA AGAGACGCCG GTGGTGGTGG GCGCCATTCC
GTTCGATACG CGCCGGCCTT CCTGCCTGTA CGTGCCTGAA AACTGCCAGT
TTGTCGCCAA CGACAGCTTT ACCCGCGCCG CGCGCCCGAT GCTGCAACAG
CCGCATCGCC TGGCCGCCTG CACAGCATTC CGGACGAACG CGCTTAAGCA
CGCGG



BLASTX with this sequence (full results here) gives as most significant hit :



>gi|4150886|emb|CAA70528.1|   (Y09356) isochorismate synthase [Pseudomonas fluorescens]
          Length = 391

 Score = 89.7 bits (221), Expect = 1e-17
 Identities = 49/116 (42%), Positives = 68/116 (58%), Gaps = 3/116 (2%)
 Frame = +3

Query: 570 QSTFLYRSEFRSLSADGVFERIETPAFGGEQEGSALAQHIRQALARAKAAGQETPVVVGA 749
           Q +F + S  R L+  G+ +RIETPA GG+   S   Q I QAL RA+ AGQ  P++VGA
Sbjct: 20  QRSFSFTSGDRELAVTGMLQRIETPAIGGDDANSLFQQTIAQALDRAREAGQSNPIIVGA 79

Query: 750 IPFDTRRPSCLYVPENCQFVANDSFTR---AARPMLQQPHRLAACTAFRTNALKHA 908
           IPFD   PSCLY+PE+ Q+   D   +   +  P L +   +    AF+  A++HA
Sbjct: 80  IPFDPAEPSCLYIPEHAQWRTRDIHAKTGMSTLPELIEQKNIPDEQAFK-RAVEHA 134


and also

>gi|22126465|ref|NP_669888.1|   (NC_004088) yersiniabactin biosynthesis component [Yersinia pestis
           KIM]
          Length = 218

 Score = 70.5 bits (171), Expect = 7e-12
 Identities = 39/87 (44%), Positives = 52/87 (58%), Gaps = 1/87 (1%)
 Frame = +2

Query: 2   ELWGDRFGGGREALLREGAA-VQRLLTLTFSAKESLFKALYPQVRCYFDFLDARMVAVDT 178
           E+W         +LL++G     + LTL FSAKESLFKA+YPQ   YFDF++AR+++   
Sbjct: 119 EIWSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYPQSGRYFDFIEARLLSYSL 178

Query: 179 QRQTFVLALLKTLTPNCPAGRRFNGRF 259
               F L LL+ L    PAGR F+G F
Sbjct: 179 VSGNFELQLLRHLNDEFPAGRCFSGCF 205

The transposon is thus between two genes, upstream of one homologous to pmsC and downstream of a homologue of ybtD.

The respective homologues, for example, in E. coli, (see blast results), are not adjacent genes (see the entries for entC and entD at Colibri). For Vibrio cholerae, vibC (VC0773) and vibD (VC0780) are in the same gene cluster.

These pairs of genes are involved in the biosynthesis of the siderophores enterochelin and vibriobactin, respectively. Siderophores are responsable for iron uptake, often a limiting factor for bacterial growth and virulence (see this review by Braun, for example).