10E5

JEP131 517 bp

JEP132 399 bp and then into vector


Composite sequence:

GTTACGCATA GACCGNCAGC TCACGAGNGG TATGATACCG GCNACGTCCA
GTTCACGCCT GCGAACGCAC GCGCCCGGCG GTGCTGCGTC ACGGTTTCGC
CCTCCACCAG CTCATTGACC ATCACGTTGC GTTTGGTGAT GTCGAACAGC
GCCAGCGTAC CGGTGACGCC GTTCGGCAGC GCCAGCTTGC CGCCGACCTC
CCACGACTTG CCCTGTTCCG GCGGCAGCGA ATCAATTTGG GTGGCGATGG
AAGAGTTCGG CTTGAACGAC TCGGTATAGC TGCTGTACAG NACACGTAAG
GCGTCAGCTT GTAGACCACC CCGGCTGCGC GGCACCAGCT TGNTGTCCGA
GCTGTCGGTG TTGGTCTGGA ACGGCCGCCC CTTGCCGGCA TAGACGTCGA
AAGCGTCGTA ACGCAGCCCG CCCATCACCA GCCATTTATC AGTCAATTGG
ATAGAATCCT GCATAAACCA ACCGTAGCTG GTGAGGTTTT CCCGCTGGTC
GCTTNTGGCC CCAAAGA

NTTCNCTTGG GACNCTTTGG CGCGACCGCG GTCGAGGCGG CATCAGGCCG
TATACCGGAT GATAGATATT GAAATCGCTG TTCTTTTTAC CGCGGATCAT
GTCGCCGCGG TAGGTACGGT TATCTTCAAA ATCAAAGCCG AACAGCATCT
GGTGGTTGAT GCTGCCCCAG TCCACGTCGC CATTGAGCGT CAGCTGCACC
GCGTTGGCGT GGCTTGACGG CGTTGGCGGT CGAGTCGGCC TGGCGGCTCA
ACACGCCGGT CACCGGGTTC AGCGCCGTGG CGCGCGCCTG GTTGTCACTG
TAGCTGTTGC GGCTGTAGGC GTAGGTCAGC GAGCTTTTCC AGCGCTCGTT
GAGCGTTTGA TCGATCTGCA GGCATGCAAG CTTCAGCTGC TCGAGTTCT


BLASTX with this sequence (full results here) gives:


>gi|16121259|ref|NP_404572.1| (NC_003143) putative hydroxamate-type ferrisiderophore receptor
           [Yersinia pestis]
          Length = 699

 Score =  254 bits (650), Expect(2) = 7e-82
 Identities = 124/216 (57%), Positives = 157/216 (72%)
 Frame = -3

Query: 731 SHANAVQLTLNGDVDWGSINHQMLFGFDFEDNRTYRGDMIRGKKNSDFNIYHPVYGLMXX 552
           SH+ AVQ  +NGD+DWG  +HQ++FG D+E  R +RGDMIRG KN+ FN+Y P+YG +  
Sbjct: 336 SHSQAVQWVVNGDLDWGFTHHQLMFGIDYEARRVFRGDMIRGSKNNGFNVYDPIYGTLPP 395

Query: 551 XXXXXXXXXXEXFGAXSDQRENLTSYGWFMQDSIQLTDKWLVMGGLRYDAFDVYAGKGRP 372
                         A SDQREN+ S   F+QDS++L + WL++GGLRYD+FDV AGKGRP
Sbjct: 396 STAVSA--------ADSDQRENIDSRALFIQDSMRLNEHWLLLGGLRYDSFDVMAGKGRP 447

Query: 371 FQTNTDSSDXKLVPRSRGGLQADALRVLYSSYTESFKPNSSIATQIDSLPPEQGKSWEVG 192
           F+ NTDSSD +LVPR+      +    LY SYTESFKPN SIAT ID+LPPE GKSWE G
Sbjct: 448 FKKNTDSSDSRLVPRAGVVYNLNDWSSLYVSYTESFKPNVSIATAIDALPPEYGKSWEAG 507

Query: 191 GKLALPNGVTGTLALFDITKRNVMVNELVEGETVTQ 84
            K+ + N VTGTLA++DI KRN+MV+ELV+GETVT+
Sbjct: 508 YKIDINNRVTGTLAVYDIHKRNIMVSELVDGETVTR 543


 Score = 72.0 bits (175), Expect(2) = 7e-82
 Identities = 34/49 (69%), Positives = 40/49 (81%)
 Frame = -2

Query: 882 IDQTLNERWKSSLTYAYSRNSYSDNQARATALNPVTGVLSRQADSTANA 736
           I+  LNE W + L YA+SRNSYSDNQARATA N  TGVL+RQ+D+TANA
Sbjct: 286 INHDLNEFWSTQLNYAFSRNSYSDNQARATAFNAETGVLTRQSDATANA 334

The transposon is thus inserted within with a gene that encodes a homologue of a putative hydroxamate-type ferrisiderophore receptor (YPO0956).

STRING analysis suggests that neither YPO0956 nor its orthologs occur repeatedly with certain genes in one gene cluster.

In the Pseudomonas aeruginosa Community Annotation Project, one can see that the Pseudomonas homologue PA0151 contains a TonB dependent receptor C-terminal region. One hundred and fourty nine base pairs upstream from the orf annotated PA0151, two genes were identified with similarity to the genes encoding PupI and PupR in Pseudomonas putida (PA0149 and PA0150, respectively). PupI belongs to the family of extracytoplasmic-function (ECF) sigma factors and PupR is a transmembrane regulator. In P. putida, PupI and PupR controlled expression of the gene encoding the siderophore receptor PupB. Briefly, in the absence of the siderophore, PupR binds PupI and prevent transcriptional activation of the pupB gene. Binding of siderophores to the PupB receptor induces its interaction with PupR, wich in turn release PupI and allows upregulation of the pupB gene. The genetic organisation of PA0149, PA0150 and PA0151 is very similar to pupI, pupR and pupB. Indeed, the stop and start codons of pupI and pupR overlaps similarly as PA0149 and PA0150, whereas pupB is located 86 bp downstream pupR.

TonB-dependant receptors are required for virulence in different systems, including uropathogenic Escherichia coli, Shigella dysenteriae, and Pseudomonas aeruginosa.

orbA, that encodes a homologous protein (see BLAST2 result), is required for ferric ornibactin uptake in Burkholderia cepacia and has been shown to be important in the pathogenesis of B. cepacia respiratory infections