8H1

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:


JEP131 411 bp and then into vector


JEP132 800 bp


 
Composite sequence:


ATATCATCGA TGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC
CTGCAGCCGG TGCGCATCGC CGGCGTCGAG CTGGGCGGCA TTCCGGCCGA
TATTCCGCTG GCCTCGTTGG GGCTGCAGGC GCTGAACGGC GTGGCCTTGC
GGCTGCAATC CTTGCGCAGC GCCGATATCT CCAGCCCGGG GCAATTGGAA
GTGTGCGCGG AAATTACCGC CACGCCGGCC GCGCAAAAAG AAATTGAGGG
TATTGTCTGC CGCGTCAGCC TGGAGAAAAG CGTGAGTGCC GTTCGCTGGC
GCGTCGCGTC CGAATTACCG GTCTGATTAA TTATATAAAT ATTTATTAAC
TTGATTATAT GCTCAGGTTA TTCGGGCTCG CCTGAATAAC GCGGAGCATT
AAGGAGGCGT T

AGAAGGACGA TGACCCCGGA AGGAAAATGA TTCCAGCGTG GCTGACAGGG
TAATTCCGCC ATTTAGTCAC GCTAATAAAA AATTCCAAGC TGCGCAGAGT
CACCTGTTTA TCGGGCGGGG CTTCGCTGTG CGCAGAACGA AAATAAGAGA
ATAGCGTTAT GACTCAGATT AATGAAAGAA CGCGTCGCCG TGATAAAGAC
GCGGTGACCT ACGCCATTGC GCAAGAGGCG ACCAACAGCA TCGCTCAAAC
GCTGGCGAAC CTGAAGTGCA ACCGCAACGG CTTGACCCAG GACGACGCCG
ATGAGCGGCT GGAACAGTTC GGCGCCAATC AGGTCGCCCA TGACAAGGCG
CCGCATGCCC TGATCCAGCT GATCAAGGCC TTCAACAACC CGTTTATCTT
CGTGCTGATG GTGCTGGCTG CGATCAGTTT CTTTACCGAT TATTGGCTGC
CGCGCCAGAG CGGTGAAGAA ACTGAACTCA CCGGCGTCAT TATCATCCTG
ACCATGGTCA CGCTGAGCGG GCTGCTGCGT TTTTGGCAGG AATACCGCAC
CAACAAAGCG GCGGAAGCGT TGAAGTCGAT GGTGCGCACC ACCGCCACGG
TGCTGCGCCG CAGCAGCTAC AGCGCGCATC CGCTGACGCT GGAAGTGCCG
ATCCGCGAAC TGGTGCCGGG GGATATCATC CAGCTCTCCG CCGGTGATAT
GGTGCCGGCC GACGTACGCC TTATCGCCTC GCGCGATCTG TTCATCAGCC
AGGCGATTTT GACCGGCGAA GCGATCCCGA TCGAGAAATA CGATGCCATG



BLASTX with this sequence (full results here) gives as most significant hit :


>gi|16121926|ref|NP_405239.1| (NC_003143) Mg(2+) transport ATPase protein B [Yersinia pestis]
           Length = 899

 Score =  293 bits (751), Expect = 5e-79
 Identities = 148/214 (69%), Positives = 173/214 (80%)
 Frame = +3

Query: 570  MTQINERTRRRDKDAVTYAIAQEATNSIAQTLANLKCNRNGLTQDDADERLEQFGANQVA 749
            M ++ +    R  +   +AIA EA NS+ QTL  L  + NGLT++DA ERLE +G NQVA
Sbjct: 1    MRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNGLTEEDARERLELYGINQVA 60

Query: 750  HDKAPHALIQLIKAFNNPFIFVLMVLAAISFFTDYWLPRQSGEETELTGVIIILTMVTLS 929
            H+KAP A +QL+ AFNNPFIFVL++LA+ISFFTDYWLP Q G ET+L GV II+TMV +S
Sbjct: 61   HEKAPPAFLQLLAAFNNPFIFVLIILASISFFTDYWLPLQEGGETDLIGVSIIVTMVLIS 120

Query: 930  GLLRFWQEYRTNKAAEALKSMVRTTATVLRRSSYSAHPLTLEVPIRELVPGDIIQLSAGD 1109
            GLLRFWQEYRTNKAAEALKSMVRTTATVLRR  Y A P   E+PI +LVPGDII LSAGD
Sbjct: 121  GLLRFWQEYRTNKAAEALKSMVRTTATVLRRGHYGAKPTQQEIPINQLVPGDIILLSAGD 180

Query: 1110 MVPADVRLIASRDLFISQAILTGEAIPIEKYDAM 1211
            M+PAD+RLI SRDLF+SQAILTGEAIP+EKYDAM
Sbjct: 181  MIPADLRLINSRDLFVSQAILTGEAIPVEKYDAM 214

Analysis of the individual fragments (here) shows that in fact the transposon is between two genes, orientated in the same sense. It is downstream of one homologous to mgtC and upstream of a homologue of mgtB. Both encode highly conserved putative Mg(2+) transport ATPase proteins.

In Salmonella, the two transporters occur within a Pathogenicity Island (SPI-3), and are under the control of the PhoP/PhoQ two-component system that is essential for virulence.