8H1

JEP131

TBLASTX full results

Best hit:


gi|15979570|emb|AJ414149.1|AJ414149 Yersinia pestis strain CO92 complete genome; segment 9/20
          Length = 193050

 Score =  127 bits (273), Expect = 8e-29
 Identities = 57/78 (73%), Positives = 66/78 (84%)
 Frame = -3 / +1

                                                                          
Query: 325    GIPADIPLASLGLQALNGVALRLQSLRSADISSPGQLEVCAEITATPAAQKEIEGIVCRV 146
              G   +I + +L LQALNG+AL LQSL SADI+  GQLEVCAEI A+PAAQKEIE IVCRV
Sbjct: 149893 GAEDEILVRTLILQALNGMALHLQSLCSADIARSGQLEVCAEIVASPAAQKEIENIVCRV 150072

                                
Query: 145    SLEKSVSAVRWRVASELP 92
              SLE+SVSA+RWR+ASELP
Sbjct: 150073 SLERSVSAIRWRIASELP 150126



JEP132


TBLASTX full results

Best hit:


>gi|15979570|emb|AJ414149.1|AJ414149 Yersinia pestis strain CO92 complete genome; segment 9/20
          Length = 193050

 Score =  351 bits (761), Expect = 1e-95
 Identities = 151/219 (68%), Positives = 177/219 (79%)
 Frame = +3 / +2

                                                                          
Query: 144    *ENSVMTQINERTRRRDKDAVTYAIAQEATNSIAQTLANLKCNRNGLTQDDADERLEQFG 323
              *+ SVM ++ +    R  +   +AIA EA NS+ QTL  L  + NGLT++DA ERLE +G
Sbjct: 150518 *KRSVMRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNGLTEEDARERLELYG 150697

                                                                          
Query: 324    ANQVAHDKAPHALIQLIKAFNNPFIFVLMVLAAISFFTDYWLPRQSGEETELTGVIIILT 503
               NQVAH+KAP A +QL+ AFNNPFIFVL++LA+ISFFTDYWLP Q G ET+L GV II+T
Sbjct: 150698 INQVAHEKAPPAFLQLLAAFNNPFIFVLIILASISFFTDYWLPLQEGGETDLIGVSIIVT 150877

                                                                          
Query: 504    MVTLSGLLRFWQEYRTNKAAEALKSMVRTTATVLRRSSYSAHPLTLEVPIRELVPGDIIQ 683
              MV +SGLLRFWQEYRTNKAAEALKSMVRTTATVLRR  Y A P   E+PI +LVPGDII 
Sbjct: 150878 MVLISGLLRFWQEYRTNKAAEALKSMVRTTATVLRRGHYGAKPTQQEIPINQLVPGDIIL 151057

                                                     
Query: 684    LSAGDMVPADVRLIASRDLFISQAILTGEAIPIEKYDAM 800
              LSAGDM+PAD+RLI SRDLF+SQAILTGEAIP+EKYDAM
Sbjct: 151058 LSAGDMIPADLRLINSRDLFVSQAILTGEAIPVEKYDAM 151174




BLASTP with the hybrid sequence

CSADIARSGQLEVCAEIVASPAAQKEIENIVCRVSLERSVSAIRWRIASELP
KRSVMRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNG

Full results here.

Top hits:

>ref|NP_405238.1| (NC_003143) Mg(2+) transport ATPase protein C [Yersinia pestis]
          Length = 236

 Score =  103 bits (258), Expect = 7e-23
 Identities = 52/52 (100%), Positives = 52/52 (100%)

Query: 1   CSADIARSGQLEVCAEIVASPAAQKEIENIVCRVSLERSVSAIRWRIASELP 52
           CSADIARSGQLEVCAEIVASPAAQKEIENIVCRVSLERSVSAIRWRIASELP
Sbjct: 184 CSADIARSGQLEVCAEIVASPAAQKEIENIVCRVSLERSVSAIRWRIASELP 235


>ref|NP_405239.1| (NC_003143) Mg(2+) transport ATPase protein B [Yersinia pestis]
          Length = 899

 Score = 89.0 bits (219), Expect = 2e-18
 Identities = 41/41 (100%), Positives = 41/41 (100%)

Query: 57 MRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNG 97
          MRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNG
Sbjct: 1  MRKLEKTDANRSNNKKPFAIALEAKNSLEQTLFKLNTHLNG 41

The transposon is thus between two genes, homologous to mgtC and mgtB, both encoding putative Mg(2+) transport ATPase proteins.

STRING analysis of mgtC (see colour code) and mgtB (see colour code) indicates that cation transport ATPases are also found together in operons in other bacteria, but not in Pseudomonas.

In Salmonella, the two transporters occur within a Pathogenicity Island (SPI-3), and are under the control of the PhoP/PhoQ two-component system that is essential for virulence.