8G1

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:


JEP131 504 bp


JEP132 498 bp


 
Composite sequence:


NATTGCGTGG GAAGGTCCGA ACTGGANATC GCTNNTATGT AGCGAGATCA
TCAACGAGCG AGGCGGCGCC CCATGTTNTG ATTTAACATA ACCGGATTTT
CCATTCTCAG TACGTTAATG AAAGCGTCGA CACGGCCACT GAGTCGTCAG
CTATTACTTG AAAAATGTTT ACCTGAAAGC GATGCGCTCG AAAGAGTGGT
GGATACGCAT GTTTATAATC TAAGACGAAA GCTTGAAGCG GCAGGCGTTG
ATAACGTGAT TTTTAATGTG CGCGGTGTCG GCTATAGGTT TTGCAAATTA
TGAAAAGGAA AAATAAATCT CTTTTGTTTT GGGTGTCTGT TCGCATCGTC
ATCGTCGCCA TCGTGACGCT TTTTACCATT ATTCTGGCGA TGTGGGGCAT
TTTTGCTGCC CAGTATTTTT ATGTCATTCA TGAAATGCCG GCATCGGTTT
ACGAAAGATT CTTGTGCTNN CCGANACCCC AGCCTGAANT CGTTAAGAAA
NGGG

TNNNNNCCGN CAGTAGAGGC GTTCCCAGGG CTGCGATACG TATTGGGGGC
NGGATTACAG TACACCTTCN NMGNANNGAT NAAATTGGGG ATGGNTGCTT
ATTTTCCTGA TCGTNGTCGT TCCATNGATA ACGTTAATGT GCGTGCGCAT
CGCACGCCCT CTTACCGCCC AATTCCGAAA TCTCACCGAC GTTGCGGAAA
CCGTGGCTCA GGGAAATTTT AAGCAACAAG CCGAGCTGGT CCAGGCGTGT
CCTGATNNNN TAATAAGGTT CTCAACNCNN NNCAATGAGA TGGTCAATAA
ACTCGCTTNA TACGAGCGTG AAATAAAAGC GTCGCATGTG GCGTNGGCCA
TGAGTTGCGT CCACCGTTGA CCGGGGCTAT GGGCCGCCTT CAGGCATGAT
AAACAACGTG TTNCTGCGAG TCAGGCACAN CTGAATATGG GTGATGACNA
AATTAAAAAA CCTGAGTAAT CTGACNGACN ACCNGATCTC CTCTCCCC



BLASTX with this sequence (full results here) gives as most significant hits :


>gi|21243530|ref|NP_643112.1|   (NC_003919) two-component system regulatory protein [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 229

 Score = 80.5 bits (197), Expect = 8e-15
 Identities = 37/67 (55%), Positives = 50/67 (74%)
 Frame = +1

Query: 91  TGFSILSTLMKASTRPLSRQLLLEKCLPESDALERVVDTHVYNLRRKLEAAGVDNVIFNV 270
           T F++L+ L+K   +  +R  LL+ CLP+SDALERVVD+H++NLRRKLE  G+  V+  V
Sbjct: 162 TEFNLLALLLKTPFKAFTRSELLDACLPDSDALERVVDSHIHNLRRKLEVHGITGVLVTV 221

Query: 271 RGVGYRF 291
           R VGYRF
Sbjct: 222 RAVGYRF 228



>gi|21243531|ref|NP_643113.1|   (NC_003919) two-component system sensor protein [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 397

 Score = 47.0 bits (110), Expect(2) = 5e-07
 Identities = 39/142 (27%), Positives = 57/142 (39%), Gaps = 7/142 (4%)
 Frame = +2

Query: 449 LRKILVLXXTPA*XR*EXGXX-RQ*RRSQG-----CDTYWGXDYSTPSXXXXNWGWXXXX 610
           LR  L+L   PA  R E      Q R +Q         Y+  +   P     +W W    
Sbjct: 45  LRYSLILSNVPAEPRAEISQLIEQPRANQARLWELFQRYYDIENFLPGLANPDW-WMLGA 103

Query: 611 XXXXXXXXXXMCVRIA-RPLTAQFRNLTDVAETVAQGNFKQQAELVQACPDXXIRFSTXX 787
                      C  +A RPL+ QF  +   A  V+ G+F  +A++V+  PD     +   
Sbjct: 104 MMTISVPVILFCGLLASRPLSRQFSQVAFAAHRVSDGDFGARAQVVRGAPDELTALADDF 163

Query: 788 NEMVNKLAXYEREIKASHVAXA 853
           N M  +L  YERE++ S    A
Sbjct: 164 NTMSGRLQQYERELRDSSAMLA 185



 Score = 36.6 bits (83), Expect = 0.13
 Identities = 15/46 (32%), Positives = 25/46 (53%)
 Frame = +3

Query: 306 RKNKSLLFWVSVRIVIVAIVTLFTIILAMWGIFAAQYFYVIHEMPA 443
           R    L +WV +R+  +A+VT+  I   MW  F  +Y  ++  +PA
Sbjct: 11  RNQAPLWWWVGLRMSALAVVTMMVIAFGMWCYFNLRYSLILSNVPA 56


 Score = 27.3 bits (59), Expect(2) = 5e-07
 Identities = 11/17 (64%), Positives = 13/17 (75%)
 Frame = +1

Query: 847 VGHELRPPLTGAMGRLQ 897
           + HELR PL  AMGR+Q
Sbjct: 184 LAHELRTPLNAAMGRVQ 200

The transposon is thus inserted in a gene that encodes a homologue of a two-component system sensor protein (see the Genbank entry for Xanthomonas axonopodis baeS).

This gene is downstream of a gene that encodes a homologue of a two-component system regulatory protein. This arrangement is conserved in Xanthomonas axonopodis, where the pair of genes are annotated as baeR and baeS, respectively. But in E. coli the order of these two is reversed (baeS being upstream of baeR; see the entry for baeS at Colibri), and this order is conserved for the orthologous proteins in multiple species (see the entry for this class of signal transduction histidine kinase at STRING). So the Xanthomonas axonopodis pair are probably not real baeR and baeS orthologues and there appears to be no functional information on this particular two-component system. On the other hand, it is interesting to see that the pair of genes immediately upstream of yeqS/b2086, are baeS and baeR (see entry for clone 8E2).