8C7

Site of transposon insertion cloned as PstI fragment into pSP72. 
Sequenced in opposite orientations with JEP131 and JEP132,
from sites at opposing ends of the transposon, to give:

JEP131 411 bp

JEP132 428 bp

 
Composite sequence:

GGAACAGGGG CTGAATGACG ACGAAACCGC CATCGCCGAC GACGCTCTGG
ATTTGATGGC TCGTCTCATC CACGGCTTCA GGTTCATTAA TCTAGAGGGT
GAAGAGGACA GTCCCCATGC ATTGGTTGAA TTTCAAGCGC TACAAGAGTG
ACGTTGCCAA ACAGGCCGTG CCGCCGCATT TGAATGCGGC CGAATTTGCA
CGGCATTACG CCGATAAGCC GCAAACCGAT ACGGAAGAGT ATCTTTCGCT
CAGCGGGGAG ATGTGCTGGG ATGCGGTGGT GCTTTGTGCC CACCGTTCTG
GCGCGCTCAG CAAGGCGAAA TACAAGCAGT TGTGGCAGAC GGTGTTCGAT
AAGCAATACA AACATTTCGT TAGCCCGGAT GATNCCGAGA TCCGCACTAT
GGCCGGTANC C

GANNCCCGNA TGCTGCGCGC GCCGCAGGGA TGTTTTATCG GCATATTCTC
CCTGCGCGAC GCGGCTGCGC CCCGTTTGCT GCATGCCATG ATCGGTACCG
GTGCGGGATT TGCCGCAGGC AATAAAAATC TTTGCATCGG CGTGGGGGGC
GCGGTCGGCT GGGAAAATCT CAATCTGGCG CGCGATCTGC GCTGGCAGCC
GGAAGGCGGT TTTTTACGCC AGGGTGATAA CGAAGTGCTG CGCATTTTTT
ATCGGCCTTT CCCCGCCTGA GCCGGTCCGG ATGCTTTTAG AGTAAAAAAA
CCGCCGTCTG GCGGTTTTTT GTTGGCGCGA ATCCCCCTTA TTTAAACCCG
CCGTTGCGAA TGAATTCAAG GCCGGCGGCG GTGATCCGCG TTTGATGCGG
GTCGATATCA TAGTTGTTCT CGTCGATG

BLASTX gives no signifcant hits and while with TBLASTX a number of very marginally significant hits are obtained (full results here), in all cases the different alignments are not in the correct reading frame.