Proteomics

The CIML proteomics platform is a platform for the IFR137 (Institute of Oncology and Immunology of Marseille), common to the UMR599, Institute Paoli-Calmettes and the CIML.
This platform is working with researchers from these sites and three outside laboratories to carry out projects in proteomics to identify and characterize proteins of interest, whether these are new protein complexes or the proteins whose expression is altered during experimental tests.

It is equipped for complete separation and characterization of proteins:

• Qualitative and quantitative two-dimensional electrophoresis (2D-DIGE technology)
• System image acquisition Ettan Dige fluorescence imager with analysis software (Decyder 7; Samespot).
• PLC Xcise (Shimadzu) used to acquire images of gels, cutting spots, tryptic digestion and deposition on Maldi plates.
• 2 mass spectrometers for the identification and characterization of proteins:
• Maldi-TOF-TOF
• ion trap coupled to a system of capillary nanochromatography.

In 2008, the platform helped set up a new structure for the platforms of the city of Marseille: MaP (Marseille Proteomics). This new structure has been validated by IBiSA 2008.