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IM2V

Molecular Imaging in a Live Environment

The platform IM2V has received the INCA label. It has been installed at the CIML since 2008. It is managed by Sebastian Mailfert under the scientific direction of Didier Marguet. IM2V offers a set of advanced techniques based on light microscopy to quantify elements relevant for the analysis of diffusion processes at the molecular level and possible interactions between different partners.

The platform provides scientific and technical assistance to implement and interpret experimental data under the best experimental conditions.

IM2V acquisition offers the following positions:

Poste Description/Application

Spot variation FCS and spot FRAP

 Fluorescence correlation spectroscopy at different spot of observation (svFCS) & Fluorescence recovery after photobleaching (spot FRAP)
⇨ Diffusion of fluorescently labeled molecules in living cells
FCCS Fluorescence cross correlation spectroscopy (FCCS)
⇨ Molecular interactions in living cells
Single molecule imaging Following individual molecules at high density
⇨ Dynamic map of the fluorescent molecules diffusion in living cells
Holographic optical tweezers Optical tweezers for the simultaneous manipulation of samples (cells, beads, etc.) coupled with fast confocal fluorescence imaging
⇨ Cell-cell interactions
Nanoscopy High spatial resolution multimodal imaging
⇨ Quantitative study of the molecule distribution in biological samples
RAMAN Imagery by RAMAN spectroscopy
⇨ Chemical composition of biological samples (i.e. application to cancer diagnosis)
Phase imaging Phase contrast imaging (PHASICS) combined with fluorescence microscopy