MultiSite(tm) Gateway
MultiSite(tm) represents the latest development
of the Gateway®
Cloning Technology. Using multiple att-site specificities and
high
efficiency of Gateway site-specific recombinational cloning it
is now
possible to clone multiple DNA's into a single vector background,
thereby
opening further fields of application such as the co-expression
of multiple
proteins from a single construct, rapid screening for the effects
of various
promoters and tags, rapid assembly of knock-out constructs, pathway
engineering etc.. This technique may be used to fuse DNA's or
to clone
multiple individual DNA's. This presentation will provide an
overview of
the mechanism and applications of the MultiSite(tm) Gateway®
Technology.