Db11-derived mutants attenuated during the infection of D. melanogaster

 

 Weakly attenuated

 Attenuated

 Strongly attenuated

 8E11

 7A8

 20C2

 10F7

 10H1

 21C1

 18D4

 10H4

 21C4

In each experiment, batches of at least 20 females were injected with Db11 as a positive control (closed squares) and 20 flies were injected with the selected clone (open squares). Flies that did not recover from the injection procedure were discarded. The viability of the flies was assessed at 20 °C over a period of at least 24 hours.

The curves show representative results of at least two trials.