7D1
/jep 131
TTTNTTGGCC AGGTGAAAAA CTGGCTGAAC AGTACGCAAA TAAAAATAGC
CAAGGCTCAG TTCAAGGGAC TTACCATACT TTAGATTGTC TTTCACTGCA
GGTCGACTCT AGAGGATCCC CGGGTACCGA GCTCGAATTC ATCGATGATA
TCAGATCTGC CGGTCTCCCT ATAGTGAGTC GTATTAATTT CGATAAGCCA
GGTTAACCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGCG TTTTCATAGG
CTCCGCCCCT GACNAGCATC ACAAAAATCG ACGCTNAGTC AGAGGTGGCG
AACCCACAGA CTATAAGATC CAGGCGTTCC CCTGGAAGTC CTTGTNCNTT
CTGTCGN

The last 399 bp clearly corresponds to the
cloning vector (see BLAST results).

And if one uses only the edited sequence:

TTTNTTGGCC AGGTGAAAAA CTGGCTGAAC AGTACGCAAA TAAAAATAGC
CAAGGCTCAG TTCAAGGGAC TTACCATACT TTAGATTGTC TTTCACTGCA
GGTCGACTCT AGAGGATCCC CGGGTACCGA GCTCGAATTC ATCGATGATA
TC

one gets this TBLASTX result

>gi|6526959|dbj|AB028452.1|AB028452 Bacillus sp. MS gene for Cpn10 and Cpn60, complete cds
          Length = 2834

 Score = 42.8 bits (87), Expect = 3e-04
 Identities = 16/19 (84%), Positives = 18/19 (94%)
 Frame = +1 / +3

                               
Query: 85   IVFHCRSTLEDPRVPSSNS 141
            ++F CRSTLEDPRVPSSNS
Sbjct: 2775 LLFFCRSTLEDPRVPSSNS 2831

But in fact, this is because the latter sequence has a bit of vector in it, as can be seen by BLASTN.

Which leaves one with just 95 bp.....

TTTNTTGGCC AGGTGAAAAA CTGGCTGAAC AGTACGCAAA TAAAAATAGC
CAAGGCTCAG TTCAAGGGAC TTACCATACT TTAGATTGTC TTTCA