3H5
/jep 132
GNANAGGNAT TAGCCTTAGC TGTCGCCCTC GGGGCGATCA CCTCCTTCGC
CAACGCGGCT GAAATCTACA ATAAGGATGG TAATAAACTC GACCTGTACG
GCCGCGTTAA CGCCAAGCAT TATTTCAGCG ACAGTAAGTC CAATGACGGC
GATATGACCT ATGTTCGCCT GGGCTTCAAA GGCGAAACGC AAATCAATGA
CCAGTTGACC GGTTACGGTC AGTGGGAATA CAACGTCCAG GCCAATCACG
ACGAAGCGCA GGGCACCGCC GGCACCAAAA CGCGTCTGGG CTTCGCGGGG
CTGAAATTCG GCAACTACGG TTCTTTCGAC TACGGCCGTA ACTACGGTGT
GGTGTATGAC GCCGAGGCTT ACACCGACAT GCTGCCAGAA TTCGGCGGCG
ACTCCTACAG CTTACCGATA ACTTTATGAC CGCCGTTCAA CGGTTTGGCT
CCTACCGTAC CCGNGATTTC TTCGGTTGGN GGAAGGCTGA NNTGCGGNGN
NNNTAGGNAA AACAT

The abrupt discontinuity obtained upon TBLASTX analysis with this sequence (results here) indicates that more than one fragment is present in the clone. This was confimed by restriction digest analysis. Presumably there is a Pst I site at around position 49, not found in the above sequence that comes from a single read.

The remaining sequence is:

GNANAGGNAT TAGCCTTAGC TGTCGCCCTC GGGGCGATCA CCTCCTTCG