10F7
/ jep 131
ACCCGCGGCG TCGGGCCGTT GGCTGCCCAG CCCGACGGCG
ACGCGCGCAT
CCCAGTGCGC GGCGCCCATC GCCCGCAGCG CGAGGCGAAT ATATACCGCC
AGCAGCAGCC CGTCCTCCGG GGCGGCCTGC GCCTGGAAGG CGTCGCCGCG
AAAGATTTCC ACCTGATGAA GCTGCCCGTC GCGCTGCAGC TGAGCGGCAA
CCAACCGCTA AGGCCGGAGC ATTGCCATAA CCCGGTGTGC AGCGCCTGCC
TGAACGACAG CCACCGCCAG CATCCCGACA ATCCGTCGCT GAGCTGGAGC
CTGAGCGACA AGCTCGGCGA CTACGGCGTG ATCGTGGCAC AGCATGCGCC
GCAGCAACCG CTGAACGATG AACATCGCCA ACTGATCAAT ACCCTGGTGG
AACAGCTGAC CAGCGTGCTG GCCATCGAGC GTCAGGTCGA CCACCAACAG
CAGCTGATGC TGATGGAGGA GCGGGCGGCG ATCGCCCGTG AGCTGCACGA
CTCCATCGCC CAATCGCTCT CCTGCCTGAA AATGCAGATC GCCTGTCTGC
AGGGCGATGA AACCGGCAAA AACGAACTGG AAAGCCGACT GGAAGAGGGC
TGTTATTTCT TGCTGTTCGA TCAGCGTTCC ACGTTGGCCG CCGACCCCGC
CATTCGCAGC TGGCTGTGCG AATTGATCAA CCCCATTTCA CTGCTGGGCC
ACGCCGGGCC GCTCTACCAG CGCTATTGGT CGCCGGCATA CGGCCTGCTG
CCGCGCTGGC ACCACAACCG CACGCTGGCG CAGCAAC
Several PstI fragments have been ligated to the Tn5-containing fragment as can be seen by the TBLASTX analysis here, wherein, for example, the site at position 551 is confirmed.
This leaves the first 286 bp:
ACCCGCGGCG TCGGGCCGTT GGCTGCCCAG CCCGACGGCG
ACGCGCGCAT
CCCAGTGCGC GGCGCCCATC GCCCGCAGCG CGAGGCGAAT ATATACCGCC
AGCAGCAGCC CGTCCTCCGG GGCGGCCTGC GCCTGGAAGG CGTCGCCGCG
AAAGATTTCC ACCTGATGAA GCTGCCCGTC GCGCTGCAGC TGAGCGGCAA
CCAACCGCTA AGGCCGGAGC ATTGCCATAA CCCGGTGTGC AGCGCCTGCC
TGAACGACAG CCACCGCCAG CATCCCGACA ATCCGT
And there is a PstI site at position 188 that separates regions of homology in the TBLASTX search with the above fragment. So it's probably real, leaving: ACCCGCGGCG TCGGGCCGTT GGCTGCCCAG CCCGACGGCG ACGCGCGCAT CCCAGTGCGC GGCGCCCATC GCCCGCAGCG CGAGGCGAAT ATATACCGCC AGCAGCAGCC CGTCCTCCGG GGCGGCCTGC GCCTGGAAGG CGTCGCCGCG AAAGATTTCC ACCTGATGAA GCTGCCCGTC GCGCTGCA