Each nylon filter was hybridised with a P33-lablled oligonucleotide probe (corresponding to the T7 primer) and subsequently hybridised with a complex probe derived from labelled reverse-transcribed total RNA extracted from worms under the test conditions. The radioactivity was quantified as photostimulated luminescence (PSL) (Fuji BAS system) for each spot.

The raw data from two independent experiments are in TableI.xls and TableII.xls. A value of '1' indicates that no signal was detected.

In the first column, the Spot name, the cDNA group to which the cDNA belongs, and the actual cDNA used are shown. Further details for each clone can be found at the Kohara lab cDNA database.

In subsequent columns, the PSL values obtained for each spot with the complex probe are given for control worms at 24 h (OP50 @24 h), for the corresponding value obtained for each spot with the oligo probe on the same filter (ov (OP50 @24 h)), with the complex probe from infected worms at 24 h (Db11 @ 24 h), etc.

The PSL value obtained for a given clone with the complex probe was normalised for DNA deposition by dividing it by the corresponding value obtained for the same clone with the oligo probe. 24hOP50corr is the corrected value for control worms at 24 h, 24hDb11corr is the corrected value for infected worms at 24 h, etc.

The corrected value for the signal obtained on Db11 was divided by the corrected value on OP50, to give 24hcalc and 48hcalc.

The raw data together with intermediate steps in the calculation are in TableIModif.xls and TableIIModif.xls.

The final ratios, giving the level of induction (>1) or repression (<1) after 24 h and 48 h of transfer to Db11 are in TableIModifMin.xls and TableIIModifMin.xls.

The data was then filtered to retain only those clones that had a raw signal intensity at 24 h with the complex probe of >40 PSL in the presence of Db11 (to eliminate clones giving a signal potentially below the background level), and a ration Db11/OP50 greater than 2 at 24 h. The clones corresponding to these criteria are in TableI24gr40_2X.html and TableII24gr40_2X.html.

The data was also filtered to retain only those clones that had a raw signal intensity at 48 h with the complex probe >40 PSL in the presence of Db11 (to eliminate clones giving a signal potentially below the background level), and a ration Db11/OP50 greater than 2 at 48 h. The clones corresponding to these criteria are in TableI48gr40_2X.html and TableII48gr40_2X.html.

TableI24gr40_2X.html and TableII24gr40_2X.html were then filtered to retain only those clones that had a ration Db11/OP50 greater than 2 at 48 h, to give TableI24gr40_2X_48_2X.html and TableII24gr40_2X_48_2X.html.

Similarly, TableI48gr40_2X.html and TableII48gr40_2X.html were filtered to retain only those clones that had a ration Db11/OP50 greater than 2 at 24 h, to give TableI48gr40_2X_24_2X.html and TableII24gr40_2X_48_2X.html.

The list of clones found in both TableI24gr40_2X_48_2X.html and TableI48gr40_2X_24_2X.html is given in TableICommon_2X.html.

Similarly, the list of clones found in both TableII24gr40_2X_48_2X.html and TableII48gr40_2X_24_2X.html is given in TableIICommon_2X.html.

Two clones (highlighted in yellow) are found in both TableICommon_2X and TableIICommon_2X: yk263b10 and yk576f2

The list of clones found in either TableI24gr40_2X_48_2X.html or TableI48gr40_2X_24_2X.html is given in TableITotal_2X.html.

Similarly, the list of clones found in either TableII24gr40_2X_48_2X.html or TableII48gr40_2X_24_2X.html is given in TableIITotal_2X.html.

Two additional clones (highlighted in pink) are found in both TableITotal_2X and TableIITotal_2X: yk578h7 and yk66e9

Two additional clones (highlighted in light blue) are found in TableITotal_2X and show an induction of >2-fold at both 24 h and 48 h in the second experiment (see extract from TableIIModif.xls below): yk377g8 and yk385e2

One additional clone (highlighted in light blue) is found in TableITotal_2X and shows an induction of >2-fold at both 24 h and 48 h in the first experiment (see extract from TableIModif.xls below): yk308a12

Spot name-cDNA group-cDNA	24hOP50	24hOP50ov	24hDb11	24Db11ov	48hOP50	48hOP50ov	48hDb11	48hDb11ov	24h	48h
09I06-CELK06711-yk377g8	1	33.8	16.5	37.6	1	36.9	37.3	36.1	14.8	38.1
09P14-CELK02835-yk385e2	1	65.4	15.6	98.6	1	83.1	38.7	60.4	10.3	53.3

Spot name-cDNA group-cDNA	24hOP50	24hOP50ov	24hDb11	24Db11ov	48hOP50	48hOP50ov	48hDb11	48hDb11ov	24h	48h
07M11-CELK03788-yk308a12	1	30.6	14.2	29.1	5.3	34.1	36.7	28.3	14.9	8.4

An additional 10 clones (highlighted in light green), not present in either Table 1 or Table 2 of the paper, are found in both experiments to have a raw signal intensity at 24 h with the complex probe >40 PSL and to show an induction of >2-fold at 24 h (see TableI_II24gr40_2X.html). At 48 h there are 7 such clones (see TableI_II48gr40_2X.html)